Reporter

Part:BBa_K2611007:Design

Designed by: Yibing Tao   Group: iGEM18_SCU-China   (2018-10-07)


T7 promoter-RFP-J23100-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 907
    Illegal NheI site found at 930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1605


Design Notes

All sequence are obtained from the previous parts that can be functional in the bacterial, since there's no need for coden optimizing. The sequence of the reporter plasmid(BBa_K2611007) is used as a reference for our sgRNA design.


Source

The section of J23100-GFP-pSB1C3 comes froms the previous exsited part: BBa_J364007 in 2018 interlab study test device 4. And the section of T7 promoter-RFP comes from another previous part in the registry. However, the origin part T7 promoter-RFP do not has a terminater, therefore, we added the terminater (BBa_B0010, BBa_B0012) to it so it can be our used.

References